Volumetric Imaging of Neural Activity by Light Field Microscopy

Lu Bai1,2 • Zhenkun Zhang1,2 • Lichen Ye1 • Lin Cong1 • Yuchen Zhao1,2 • Tianlei Zhang1,2 • Ziqi Shi1,2 • Kai Wang1,2,3

1 Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China

2 University of Chinese Academy of Sciences, Beijing 100049, China

3 Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai 201210, China



    Recording the highly diverse and dynamic activities in large populations of neurons in behaving animals is crucial for a better understanding of how the brain works. To meet this challenge, extensive eforts have been devoted to developing functional fluorescent indicators and optical imaging techniques to optically monitor neural activity. Indeed, optical imaging potentially has extremely high throughput due to its non-invasive access to large brain regions and capability to sample neurons at high density, but the readout speed, such as the scanning speed in twophoton scanning microscopy, is often limited by various practical considerations. Among diferent imaging methods, light feld microscopy features a highly parallelized 3D fuorescence imaging scheme and therefore promises a novel and faster strategy for functional imaging of neural activity. Here, we briefy review the working principles of various types of light feld microscopes and their recent developments and applications in neuroscience studies. We also discuss strategies and considerations of optimizing light feld microscopy for diferent experimental purposes, with illustrative examples in imaging zebrafsh and mouse brains.




    Light feld microscopy; Volumetric imaging; Brain activity; Calcium imaging; Voltage imaging