Department of Nautical Medicine, Laboratory of Stress Medicine, Second Military Medical University, Shanghai 200433, China

Abstract 

Objective 
To examine the effect of neuropeptide Y (NPY) on TGF-β1 production in RAW264.7 macrophages. 
Methods 
Enzyme linked immunosorbent assay (ELISA) was used to detect TGF-β1 production. Cell counting kit 8 (CCK-8) was used to assay the viability of RAW264.7 cells. Western blot was used to detect the phosphorylation of PI3K p85. 
Results 
NPY treatment could promote TGF-β1 production and rapid phosphorylation of PI3K p85 in RAW264.7 cells via Y1 receptor. The elevated TGF-β1 production induced by NPY could be abolished by wortmannin pretreatment. 
Conclusion 
NPY may elicit TGF-β1 production in RAW264.7 cells via Y1 receptor, and the activated PI3K pathway may account for this effect.

Keywords

neuropeptide Y; TGF-β1; phosphoinositide-3 kinase; RAW264.7 cells

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