1 School of Life Science, Shanghai University, Shanghai 200444, China
2 Shanghai Institute of Biological Science, Chinese Academy of Science, Shanghai 200031, China

Abstract 

Objective 
To examine the effect of deglycosylation on gating properties of rNav1.3. 
Methods 
rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. 
Results 
Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. 
Conclusion 
Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.

Keywords

rNav1.3; voltage-gated sodium channel; glycosylation; two-electrode voltage clamp; Xenopus oocyte

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