Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Abstract 

In this study, we extracted total RNA from 15 intracranial aneurysms and 17 superficial temporal artery samples, then performed genome-wide expression profiling using the Affymetrix U133 Plus 2.0 GeneChip. Genes that were differentially expressed between intracranial aneurysms and arterial samples were identified using significance analysis for microarrays, and the expression patterns of three randomly-selected genes were verified by real-time polymerase chain reaction analysis. We identifi ed 3 736 differentially-expressed genes out of the 47 000 assayed transcripts. A total of 179 genes showed a >10-fold change in expression between the aneurysms and the arterial samples. Genes involved
in the proliferation, migration, and apoptosis of vascular muscle cells, atherosclerosis, extracellular matrix disruption, and inflammatory reactions
were associated with the formation of intracranial aneurysms. There were no significant differences in gene expression profile between unruptured and
ruptured aneurysms.

Keywords

intracranial aneurysms; microarray; gene expression profiles

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