1Department of Neurology, Zhongshan Hospital and Shanghai Medical College; State Key Laboratory of Medical Neurobiology; Institute of Brain Science, Fudan University, Shanghai 200032, China
2School of Life Sciences, Fudan University, Shanghai 200433, China
3Xinxiang College of Medicine, Xinxiang 453003, China
*These authors contributed equally to this work.
Co-corresponding authors: Chunjiu Zhong and Lirong Jin. E-mail: zhongcj@163.com; jinlr99@163.com

Abstract 

Our previous studies have demonstrated that ceruloplasmin (CP) dysmetabolism is correlated with Parkinson’s disease (PD). However, the causes of decreased serum CP levels in PD patients remain to be clarified. This study aimed to explore the potential association between genetic variants of the CP gene and PD. Clinical features, serum CP levels, and the CP gene (both promoter and coding regions) were analyzed in 60 PD patients and 50 controls. A luciferase reporter system was used to investigate the function of promoter single-nucleotide polymorphisms (SNPs). High-density comparative genomic hybridization microarrays were also used to detect large-scale copy-number variations in CP and an additional 47 genes involved in PD and/or copper/ iron metabolism. The frequencies of eight SNPs (one intronic SNP and seven promoter SNPs of the CP gene) and their haplotypes were significantly different between PD patients, especially those with lowered serum CP levels, and controls. However, the luciferase reporter system revealed no significant effect of the risk haplotype on promoter activity of the CP gene. Neither these SNPs nor their haplotypes were correlated with the Hoehn and Yahr staging of PD. The results of this study suggest that common genetic variants of CP are associated with PD and further investigation is needed to explore their functions in PD.

Keywords

Parkinson’s disease; ceruloplasmin; single-nucleotide polymorphism; haplotype; copynumber variation

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