Identification of autophagy signaling network that contributes to stroke in the ischemic rodent brain via gene expression
1Department of Vascular Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
2Department of Vascular Surgery, Shanghai Pudong Hospital, Shanghai 201399, China
Corresponding author: Bo Yu. E-mail: yubo120@hotmail.com
Abstract
Autophagy plays a vital role in cerebral ischemia and may be a potential target for developing novel therapy for stroke. In this study, we constructed an autophagy-related pathway network by analyzing the genes related to autophagy and ischemic stroke, and the risk genes were screened. Two autophagy-related modules were signifi cantly up-regulated and clustered to influence cerebral ischemia. Besides, three key modular genes (NFKB1, RELA, and STAT3) were revealed. With 5-fold cross validation, the ROC curves of NFKB1, RELA, and STAT3 were 0.8256, 0.8462, and 0.8923. They formed a complex module and competitively mediated the activation of autophagy in cerebral ischemia. In conclusion, a module containing NFKB1, RELA, and STAT3 mediates autophagy, serving as a potential biomarker for the diagnosis and therapy of ischemic stroke.
Keywords
cerebral ischemia; autophagy; functional module; stroke; gene expression profile
