A Novel Long-range Excitatory Neural Circuit from the Magnocellular Red Nucleus to Spinal Dorsal Horn Facilitates Neuropathic Pain-like Behaviors in Male--Neuroscience Bulletin

A Novel Long-range Excitatory Neural Circuit from the Magnocellular Red Nucleus to Spinal Dorsal Horn Facilitates Neuropathic Pain-like Behaviors in Male

Jiali Shi1,2 · Yinfeng Yuan1 · Yuhao Luo1 · Yue Guo1 · Jiashu Lian3 · Lin Lin1 · Danni Chen1 · Qian Wang1 · Xiumin Xue1 · Zhichao Chen1,2 · Yongjie Wang1 · Zhihui Huang1

1 School of Pharmacy, Hangzhou Normal University, Hangzhou 311121, China

2 Department of Pharmacy, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China

3 Department of Ultrasound, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou 310016, China

Abstract

Neuropathic pain arises from a primary lesion or disease affecting the somatosensory system; however, the brain circuitry underlying the regulation of neuropathic pain remains unclear. The red nucleus (RN) is a critical brain region involved in regulating muscle tension, coordinating movement, and facilitating sensorimotor integration. While some evidence has shown that RN regulates pain, its specific role in neuropathic pain and associated neural circuits remains elusive. In this study, we found that CaMKIIα-positive neurons in the magnocellular red nucleus (RMC^CaMKIIα) were activated following common peroneal nerve (CPN) ligation-induced chronic neuropathic pain in adult male mice. Interestingly, chemogenetic and optogenetic inhibition of these RMC^CaMKIIα neurons alleviated the thermal hyperalgesia and mechanical allodynia in the neuropathic pain model mice, whereas activation of these neurons sufficiently induced the mechanical allodynia and thermal hyperalgesia in naïve mice. Trans-synaptic viral tracing studies further revealed that long-range CaMKIIα⁺ neuron projections from RMC to the dorsal horn (DH) facilitated the neuropathic pain-like behaviors following CPN ligation. DH neurons received direct innervation from RMC^CaMKIIα neurons, and inhibition of RMC^CaMKIIα-DH^CaMKIIα circuits alleviated the neuropathic pain in the ligated mice. Taken together, these results identify a novel long-range excitatory neural circuit from RMC to DH that facilitates the neuropathic pain-like behaviors in adult male mice, providing a new target for neuropathic pain treatment.

Keywords

Neuropathic pain; Magnocellular red nucleus; Spinal dorsal horn; Neural circuit; Optogenetics; Chemogenetics

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